Send Inquiry

Request To Call Back

HotStart Taq DNA Polymerase

Get a Price/Quote

MOQ : 5 Pieces

HotStart Taq DNA Polymerase Specification

Storage Instructions
Store at -20C, avoid repeated freeze/thaw cycles

Function
Amplifies specific DNA sequences

Instruments Type
Molecular Biology Reagent

Features
Hot Start activity, high specificity, reduced non-specific amplification, easy master mix formulation

Usage Type
Laboratory usage

Measurement Range
From 0.1 ng to 1 g DNA template (dependent on protocol)

Accuracy
High specificity for DNA amplification

Shelf Life
12-24 months (when stored at recommended temperature)

Equipment Type
HotStart Taq DNA Polymerase

Material
Enzyme (Recombinant Taq DNA Polymerase), Stabilizers

Condition
New

Technology
Hot Start PCR Technology

Portable
Yes

Wall Mounted
No

Real-Time Operation
Yes

Noise Level
Silent

Operating Type
Manual reagent, compatible with automated PCR platforms

Use
Amplification of DNA in PCR reactions

Power Source
Not Applicable (Enzymatic reagent; requires thermal cycler)

Dimension (L*W*H)
Custom as per pack (typically small tube or vial)

Weight
Approx. 1-5 mL per vial (weight varies by packaging)

Color
Colorless solution

Enzyme Concentration
5 units/L

Inhibition
Reduced risk of non-specific amplification and primer-dimer formation

MgCl2 Concentration
Included in reaction buffer (typically 2-3 mM final concentration)

Application
Routine PCR, genotyping, colony PCR, DNA cloning, diagnostics

PCR Product Size Range
0.2 kb to 5 kb (typical)

Packaging
Supplied in 0.2 mL/0.5 mL/1.0 mL vials, in boxes

Hot Start Activation
Antibody-mediated inhibition, activated by initial denaturation

Buffer Supplied
10X reaction buffer with MgCl2

Unit Definition
One unit incorporates 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 74C

dNTP Compatibility
Compatible with standard dNTP concentrations (0.2 mM each)

HotStart Taq DNA Polymerase Trade Information

Minimum Order Quantity
5 Pieces

Payment Terms
Cash in Advance (CID)

Supply Ability
100 Pieces Per Week

Delivery Time
2-10 Days

Main Domestic Market
All India

About HotStart Taq DNA Polymerase

Embrace triumphant DNA amplification with our HotStart Taq DNA Polymerase-now featured in our pivotal sale and clearance event for lab essentials! Prime for high-specificity PCR, this recombinant enzyme delivers reliable performance with antibody-mediated hot start activation, reducing non-specific amplification and primer-dimer issues. Supplied in convenient vials (0.2-1.0 mL), it includes 10X MgCl2 reaction buffer and works with standard dNTPs. Accuracy and ease make it ideal for diagnostics, colony PCR, DNA cloning, and genotyping. Suitable for both routine and advanced laboratory workflows, this product is silent, portable, and supports export markets across India.

Extra Features and Material Excellence for Key Applications

HotStart Taq DNA Polymerase stands out with antibody-mediated hot start technology, ensuring each PCR run is accurate and free of non-specific artifacts. Material features include recombinant enzyme formulation with built-in stabilizers, enhancing longevity and reliability. Applications span routine PCR, genotyping, DNA cloning, and diagnostics, making it a pivotal reagent for molecular biology laboratories. Its tailored formulation supplies MgCl2 within the buffer, streamlining master mix prep for optimal results.

Sample Availability, Policy, and Delivery for Export Markets

Samples of HotStart Taq DNA Polymerase are available for export markets, supporting transparent valuation and customer satisfaction. We offer a flexible sample policy so you can evaluate product performance ahead of bulk orders. Delivery times are tailored for swift drop-off, ensuring samples and bulk shipments reach clients efficiently. Our reliable network makes this featured enzyme accessible to laboratories and research institutions nationwide and internationally, enhancing trust and supply chain performance.

FAQ's of HotStart Taq DNA Polymerase:

Q: How is HotStart Taq DNA Polymerase activated during PCR?

A: HotStart Taq DNA Polymerase uses antibody-mediated inhibition, becoming active only after an initial denaturation step in the PCR cycle to ensure specific amplification.

Q: What are the main benefits of using this enzyme in laboratory PCR applications?

A: Key benefits include high specificity, reduced non-specific amplification and primer-dimer formation, and compatibility with routine PCR, DNA cloning, genotyping, and diagnostics.

Q: When should the enzyme be stored, and under what conditions?

A: The enzyme should always be stored at -20C. To maintain its longevity (12-24 months shelf life), avoid repeated freeze-thaw cycles for optimal performance.

Q: Where can this product be used most effectively?

A: This product is ideal for research and diagnostics in molecular biology laboratories, especially those focused on routine PCR, genotyping, colony PCR, and DNA cloning.

Q: What is the process for requesting a sample of HotStart Taq DNA Polymerase?

A: You can request a sample by reaching out to our supplier or trader representatives. Upon request, delivery is coordinated to ensure timely drop-off to your laboratory.

Q: How does including MgCl2 in the reaction buffer benefit the PCR process?

A: Inclusion of MgCl2 in the 10X reaction buffer simplifies master mix preparation, provides consistency, and supports robust amplification across a wide range of DNA templates.

Tell us about your requirement